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Set A Index Adapters Consult with Index 2 (i5) Orientation To find out more on how to enter i5 bases about the sample sheet in ahead or reverse enhance orientation.
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This allows huge figures of libraries being pooled and sequenced at the same time during a single run. In comparison with other indexing procedures, one of a kind twin indexing mitigates index hopping by filtering hopped reads.
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When evaluating index sets for shade equilibrium, the sequence noticed via the instrument needs to be assessed, whatever the orientation useful for the sample sheet. For the i7 index, usually asses colour stability using the "i7 bases for sample sheet" column orientation.
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